Deciphering the metagenome-encoded pathway and domain structure for Palmerolide biosynthesis

To identify the Pal producing gene cluster (an antimelanoma gene product) in the microbial metagenome of the Antarctic ascidian, Synoicum adareanum. This entails assembling, annotating and mining the S. adareanum microbial metagenome. Then, a predictive bioorganic synthesis strategy will be applied to identify potential Palmerolide biosynthetic gene clusters. These predictions will be validated using single cell genome sequencing approach to screen candidate organism(s) gene content. 

Project Team

• Alison Murray, DRI (lead)
• Bill Baker, USF, Co-I
• Patrick Chain, LANL, Co-I
• Armand Dichosa, LANL, Senior Scientist

Funded by

DRI | IPA | NIH NCI Clinical and Translational R21